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gradient gel  (Bio-Rad)


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    Bio-Rad gradient gel
    Gradient Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+tgx+gradient+gels/10%25+Criterion+TGX+Precast+Midi+Protein+Gel/10__1002_slash_nep3__70035-100-6-13
    Average 96 stars, based on 168 article reviews
    gradient gel - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: Epigenetic and transcriptional programs define osteosarcoma subtypes and establish targetable vulnerabilities
    Article Snippet: .. Protein quantification 676 D ow nloaded from http://aacrjournals.org/cancerdiscovery/article-pdf/doi/10.1158/2159-8290.C D -25-0237/3657317/cd-25-0237.pdf by guest on 13 N ovem ber 2025 24 was performed using the Pierce BCA Protein assay kit (Thermo scientific #23227) on 4-677 15% Criterion TGX gradient gels (Bio-Rad #5671084) transferred to PVDF. .. Western 678 blot membranes were incubated in 5% milk (MP Biomedicals #902887) TBST (Bioland 679 Scientific #TBST01-10) or 5% BSA (Sigma-Aldrich #A4503) TBST for primary and 680 secondary antibodies.

    Article Title: Epigenetic and Transcriptional Programs Define Osteosarcoma Subtypes and Establish Targetable Vulnerabilities
    Article Snippet: .. Protein quantification was performed using the Pierce Bicinchoninic Acid Protein Assay Kit (Thermo Fisher Scientific, #23227) on 4% to 15% Criterion TGX gradient gels (Bio-Rad, #5671084), and proteins were then transferred to polyvinylidene difluoride membranes. .. Western blot membranes were incubated in 5% milk (MP Biomedicals, #902887) with TBS with Tween (Bioland Scientific, #TBST01-10) or 5% BSA (Sigma-Aldrich, #A4503) with TBST for primary and secondary antibodies.

    Concentration Assay:

    Article Title: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers.
    Article Snippet: .. Aliquots of the homogenates were diluted with 4X Laemmli sample buffer (Bio-Rad Laboratories) to a concentration of 0.25 μg muscle/μl and heated at 95 °C for 5 min. Proteins were separated by SDS-PAGE, 15 μl from each sample was loaded onto 26-well Criterion TGX gradient gels (4–20% acrylamide, Bio Rad Laboratories) and electrophoresis run as previously described [33, 34]. .. Proteins were transferred to PVDF membranes (Bio Rad Laboratories) and stained with MemCode Reversible Protein Stain Kit (Thermo Scientific).

    Article Title: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers
    Article Snippet: .. Aliquots of the homogenates were diluted with 4X Laemmli sample buffer (Bio-Rad Laboratories) to a concentration of 0.25 μg muscle/μl and heated at 95 °C for 5 min. Proteins were separated by SDS-PAGE, 15 μl from each sample was loaded onto 26-well Criterion TGX gradient gels (4–20% acrylamide, Bio Rad Laboratories) and electrophoresis run as previously described [ , ]. .. Proteins were transferred to PVDF membranes (Bio Rad Laboratories) and stained with MemCode Reversible Protein Stain Kit (Thermo Scientific).

    Article Title: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers
    Article Snippet: .. Aliquots of the homogenates were diluted with 4X Laemmli sample buffer (Bio-Rad Laboratories) to a concentration of 0.25 μg muscle/μl and heated at 95°C for 5 min. Proteins were separated by SDS-PAGE, 15 μl from each sample was loaded onto 26-well Criterion TGX gradient gels (4–20% acrylamide, Bio Rad Laboratories) and electrophoresis run as previously described [ , ]. .. Proteins were transferred to PVDF membranes (Bio Rad Laboratories) and stained with MemCode Reversible Protein Stain Kit (Thermo Scientific).

    Electrophoresis:

    Article Title: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers.
    Article Snippet: .. Aliquots of the homogenates were diluted with 4X Laemmli sample buffer (Bio-Rad Laboratories) to a concentration of 0.25 μg muscle/μl and heated at 95 °C for 5 min. Proteins were separated by SDS-PAGE, 15 μl from each sample was loaded onto 26-well Criterion TGX gradient gels (4–20% acrylamide, Bio Rad Laboratories) and electrophoresis run as previously described [33, 34]. .. Proteins were transferred to PVDF membranes (Bio Rad Laboratories) and stained with MemCode Reversible Protein Stain Kit (Thermo Scientific).

    Article Title: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers
    Article Snippet: .. Aliquots of the homogenates were diluted with 4X Laemmli sample buffer (Bio-Rad Laboratories) to a concentration of 0.25 μg muscle/μl and heated at 95 °C for 5 min. Proteins were separated by SDS-PAGE, 15 μl from each sample was loaded onto 26-well Criterion TGX gradient gels (4–20% acrylamide, Bio Rad Laboratories) and electrophoresis run as previously described [ , ]. .. Proteins were transferred to PVDF membranes (Bio Rad Laboratories) and stained with MemCode Reversible Protein Stain Kit (Thermo Scientific).

    Article Title: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers
    Article Snippet: .. Aliquots of the homogenates were diluted with 4X Laemmli sample buffer (Bio-Rad Laboratories) to a concentration of 0.25 μg muscle/μl and heated at 95°C for 5 min. Proteins were separated by SDS-PAGE, 15 μl from each sample was loaded onto 26-well Criterion TGX gradient gels (4–20% acrylamide, Bio Rad Laboratories) and electrophoresis run as previously described [ , ]. .. Proteins were transferred to PVDF membranes (Bio Rad Laboratories) and stained with MemCode Reversible Protein Stain Kit (Thermo Scientific).



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